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Image Search Results
Journal: International immunopharmacology
Article Title: NLRP3 Inflammasome and Glia Maturation Factor Coordinately Regulate Neuroinflammation and Neuronal Loss in MPTP Mouse Model of Parkinson’s Disease
doi: 10.1016/j.intimp.2020.106441
Figure Lengend Snippet: Increased α-Synuclein is expressed in the substantia nigra (SN) of the human PD brains. Immunofluorescence labeling of α-Synuclein from age matched non-PD and PD midbrain of SN (A). Free-floating sections were made from human post-mortem mid brain of the SN (40-μm thickness) and stained with α-Synuclein. White arrows indicate the α-Synuclein stained cells. The whole photomicrographs were analyzed using MetaMorph image analysis software to determine the integrated optical density (IOD) and total positive area. Bar graphs show the IOD (B) and total positive area (C) were quantitatively increased in the SN of the PD brains as compared with non-PD. The values are expressed as mean ± SEM of determination from each group (n=5–6). *p < 0.05 compared to non-PD brains and it was considered as statistically significant and expressed as arbitrary unit (au). Magnifications 400X and scale bar = 100 μm.
Article Snippet: The merged photomicrographs using
Techniques: Immunofluorescence, Labeling, Staining, Software
Journal: International immunopharmacology
Article Title: NLRP3 Inflammasome and Glia Maturation Factor Coordinately Regulate Neuroinflammation and Neuronal Loss in MPTP Mouse Model of Parkinson’s Disease
doi: 10.1016/j.intimp.2020.106441
Figure Lengend Snippet: Co-expression of GMF, GFAP, α-Synuclein and NLRP3 in the SN of PD brains. Double immunofluorescence labeling of GMF and α-Synuclein, GFAP in the SN of PD brain. Free-floating sections were made from human post-mortem mid brain of the SN (40-μm thickness) and stained with GMF (green fluorescence), GFAP (green fluorescence), α-Synuclein (red fluorescence) and NLRP3 (red fluorescence). Qualitatively increased expression and co-localization of GMF along with α-Synuclein (A), GFAP (B) and NLRP3 (D) were found in the SN of PD brains as compared with age and gender matched non-PD SN. The merged photomicrographs using MetaMorph image analyzing software to determine the integrated optical density (IOD) and total positive area. Bar graph shows the IOD (B) and total positive area (C) were quantitatively increased in midbrain of PD SN as compared with non-PD SN. The values are expressed as mean ± SEM of determination from each group (n=5–6/group). Magnifications 400X; scale bar = 100 μm.
Article Snippet: The merged photomicrographs using
Techniques: Expressing, Immunofluorescence, Labeling, Staining, Fluorescence, Software